The LV-DAF delivers fast, versatile autofocus with the Hybrid Auto-Focus system, making the most of two types of auto focus systems. Combining slit projection and contrast detection auto focus
, it features large focus range and fast tracking ability. A variety of observation methods are supported, including brightfield, darkfield and differential interference contrast (DIC), as well as various transparent samples.
There are two common types of auto-focus systems for microscopes: slit projection and contrast detection.
Slit projection system projects a slit image and then detects the shift in the reflected light. This system is useful when a large focal range is necessary.
Contrast detection system projects a slit pattern and then detects the contrast of the reflected light. This system is useful when focus accuracy is needed. This is possible because this auto-focus system is less affected by sample surface variation.
Hybrid Auto-Focus combines the advantages of both systems and makes the most of their paired potential.
The controller features the same hardware design as the LV-ECON and has a compact footprint that allows them to be stacked on each other and used anywhere.
The LV-DAF can be combined with other LV series products. When combined with the LV-ECON, it enables observation under the optimal conditions for each particular sample.
Nikon provides a software development kit (SDK) for integrating the LV-DAF into a variety of systems. (Compatibility is only guaranteed for Nikon products.)
The LV-DAF can be controlled from a PC or a DS-L2 digital camera system for microscopes via USB or RS232C.
The Auto-Adjustment Program, included as standard, enables simple and speedy system setup. The program performs immediate auto-adjustment after the user focuses the system and presses the button to start the setup. It is also possible to automatically set/register optimal parameters for each type of sample and recall them in accordance to the sample being photographed.
The LV-DAF uses a bright LED for the auto-focus light source. And since it also automatically adjusts the auto-focus light volume for the sample, it can support samples ranging from low to high reflectivity.
A wide range of observation methods is supported, including brightfield, darkfield, and DIC. Reflective samples and transparent samples are also supported.
Focal range is remarkably larger than with contrast detection alone. This means that samples with distortions on their surface, such as a liquid crystal substrate, can be rapidly tracked, thereby enabling speedy focusing.
| Detection system: | Hybrid system combining slit projection with contrast detection |
|---|---|
| Auto-focus light source: | Near-IR LED (λ = 770 nm) |
| Objective lens: | CFI60 objective lens 2.5x-100x (includes extra-long working distance (ELWD), super-long working distance (SLWD), and CR for LCD substrate inspection) |
| Auto-focus modes: | Continuous mode and search mode (single, continuous) |
| Focal range: | Focal range without searching (brightfield)² 2.5x: 5.5 mm or more, 5x: 4.5 mm or more, 10x: 1.3 mm or more, 20x: 320 μm or more, 50x: 50 μm or more, 100x: 10 μm or more |
| Focal time: | 0.7 seconds or less (20x: 200μm with no search)² ³ |
| Focal precision (repeated reproducibility): | 1/2 or less of focal depth² ³ |
| AF offset feature: | Enables observation with precise adjustment of focal position while applying auto-focus |
| Minimum driveresolution: | 0.05 μm¹ |
| External communication: | RS232C, USB, and parallel I/O |
| Power source: | 100-240 V AC, 1.0 A, 50/60 Hz |
¹ Some limitations for 2.5x and 100x.
² Using Nikon’s standard Cr vapor deposition sample.
³ Using the LV-IMA or LV-FMA
| Compensator: | Standard 1/4 wave and tint plate: Quartz wedge or Senarmont compensator can be inserted into intermediate slot |
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